Calc

Serial Dilutions

Question asked by Joyce:

a stock solution is diluted serially by 1 in 25, then 1ml of this solution was added to 20mL of buffer solution, what is the overal dilution factor? what is the main problem with the 2nd dilution?

Follow-up responses...

Please Clarify Your Second Question

posted by Jeffrey R Cohen:

Dear Joyce,
If you have not gotten your questions answered already, please clarify your second question, "what is the main problem with the 2nd dilution?" Then I will try to answer both questions.
Thanks, Jeff Cohen

In The Second Dilution

posted by Aminah:

when we calculate dilution, we use V1C1 = V2C2 right? The thing that we need to find out if V1 correct? In the second dilution, what is the C1 that we use? is it the amount of concentration same as the first dilution or is it the dilution factor of the first dilution?

Dilutions

posted by Nick:

To make a 1/400 dilution of 50ml of virus suspension,
Is it correct if I add,

5ml of virus in to 4.5ml of PBS then from that 5ml add to a 4.5ml of PBS. And in the end 12.5ml from the second diltion Mmix with 37.5ml of PBS?



Dilution

posted by jennie thomas:

Can you clarify this please and tell me how many mls of diluent I need.
50g is the test portion, What is a 1 in 5 suspension, followed by a 1 in 2 dilution.

SOLUTION FOR THE DILUTION PROBLEM

posted by ALFREDO PALABAY:

WHAT TO KNOW THE ANSWER TO THIS PROBLEM?

IF I HAVE A STOCK GLUCOSE STANDARD OF 600 MG/DL AND I WANT TO PREPARE THREE LEVELS OF STANDARDS HAVING A VALUE OF 300MG/DL,200MG/DL AND 100 MG/DL, HOW WOULD I MAKE THOSE DILUTIONS? WHAT DILUTION WOULD I HAVE TO MAKE OF THE STOCK STANDARD?

PLS. SHOW THE SOLUTION.

Need Help Calculating A Dilution

posted by james williams:

How would you produce a 10-1 dilution of 2.0 mL of concentrated cells using the full 2 mL volume?

please show the solution

Help

posted by John:

I have a stock solution of 12% and need a solution of 0.5%, can you please help me make this calculation. Many thanks

Dilution

posted by Piyush:

If i am adding 300 microliter of 10 micromolar atropine to 1500 microliter of water.

What should i take final volume as?
is it 1500 microliter or 1800 microliter (1500+300)
I am confused please do let me know

Serial Dilution

posted by maria:

What result are obtained from having a serial dilution of a 2ml initial concentrated solution